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    National Tsing Hua University Institutional Repository > 原子科學院  > 工程與系統科學系 > 會議論文  >  Binding efficiency improvement of antibody to antigen on mixed self-assembly monolayers

    Please use this identifier to cite or link to this item: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/47200

    Title: Binding efficiency improvement of antibody to antigen on mixed self-assembly monolayers
    Authors: Chun-Lung Wu;Yu-Wen Lin;Yi-Lin Hsu;Fan-Gang Tseng;Ching-Chang Chieng
    教師: 錢景常
    Date: 2005
    Publisher: Nano Science and Technology Institute
    Relation: 2005 NSTI Nanotechnology Conference and Trade Show. NSTI Nanotech 2005, 2005, p 359-62
    Keywords: atomic force microscopy
    molecular biophysics
    molecular orientation
    surface plasmon resonance
    Abstract: Protein chip is a potential tool on advanced disease diagnosis and drug screening, due to the capabilities of parallel process and trace-amount sample detection. The detection of proteins mainly depends on immune recognition, very sensitive to the orientation of immobilized proteins on solid surface. However, due to close packing of immobilized proteins on SAMs, the binding efficiency is usually lower than 10%. This paper proposed a novel way by employing mixed SAMs to generate nano patterns for effective separation of immobilized proteins to allow antibody conjugation from more directions. Testing results by Surface Plasma Resonance (SPR) and Atomic Force Microscope (AFM) investigation demonstrated a almost 3 fold increment on binding efficiency of protein immobilization on mixed SAMs
    URI: http://www.nsti.org/BioNano2005/showabstract.html?absno=1166
    Appears in Collections:[工程與系統科學系] 會議論文

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