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    National Tsing Hua University Institutional Repository > 生命科學院  > 生命科學系 > 期刊論文 >  Abrogation of P53 function by transfection of HPV16 E6 gene does not enhance resistance of human tumour cells to ionizing radiation

    Please use this identifier to cite or link to this item: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/49212

    Title: Abrogation of P53 function by transfection of HPV16 E6 gene does not enhance resistance of human tumour cells to ionizing radiation
    Authors: Huang H;Li CY;Little JB
    教師: 黃海美
    Date: 1996
    Publisher: Taylor & Francis
    Relation: International Journal of Radiation Biology,Taylor & Francis,Volume 70,Issue 2,1996 Aug.,Pages 151-160
    Keywords: human diploid fibroblasts
    human papillomavirus e6
    dna damage
    ataxia telangiectasia
    Abstract: Suppression of wild-type p53 expression has been shown to enhance the radiation resistance of human diploid fibroblasts, but results concerning the role of p53 expression in the sensitivity of human tumour cells have been conflicting. In order to address this question, we transfected four human tumour cell lines with the human papilloma virus 16 E6 gene and compared the radiosensitivity of subclones expressing E6 with that of subclones transfected with the neo gene alone. E6 binds to wild-type p53 promoting its degradation and abrogating its function. Two of these cell lines, one derived from a squamous cell carcinoma and the other an osteogenic sarcoma, expressed wild-type p53. The other two cell lines were of similar origins and histologies but expressed mutant or no p53 (null). Insertion of E6 into the cell was accomplished by two techniques: (1) to-transfection of plasmid vectors containing neo and E6; (2) infection with a retroviral vector containing neo and E6. Multiple transfected subclones were examined for each cell line. Transfection with E6 and abrogation of p53 function had no significant influence on the radiosensitivity of any of the cell lines tested. In particular, there was no evidence;hat loss of wild-type p53 function increased the resistance of these human tumour cell lines to ionizing radiation.
    URI: http://www.ncbi.nlm.nih.gov/pubmed/8794844
    Appears in Collections:[生命科學系] 期刊論文

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