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    National Tsing Hua University Institutional Repository > 原子科學院  > 生醫工程與環境科學系 > 期刊論文 >  Aptamer-functionalized gold nanoparticles for turn-on light switch detection of platelet-derived growth factor


    Please use this identifier to cite or link to this item: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/40766


    Title: Aptamer-functionalized gold nanoparticles for turn-on light switch detection of platelet-derived growth factor
    Authors: Huang CC;Chiu SH;Huang YF;Chang HT
    教師: 黃郁棻
    Date: 2007
    Publisher: 0003-2700
    Keywords: PROBE CAPILLARY-ELECTROPHORESIS
    SIGNALING APTAMERS
    BIOLOGICAL-FLUIDS
    FACTOR RECEPTOR
    HUMAN THROMBIN
    HIGH-AFFINITY
    DNA-BINDING
    IN-VIVO
    PDGF-AB
    FLUORESCENCE
    Abstract: ©2007 ACS - An aptamer modified gold nanoparticles (Apt-AuNPs) based molecular light switching sensor has been demonstrated for the analysis of breast cancer markers (platelet-derived growth factors (PDGFs) and their receptors) in homogeneous solutions. The PDGF binding aptamer has a unique structure with triple-helix conformation that allows N,N-dimethyl-2,7-diazapyrenium dication (DMDAP) and PDGF bindings. The fluorescence of DMDAP is almost completely quenched by Apt-AuNPs when it intercalates with the aptamers. Owing to high magnitudes of increases (up to 40-fold) in the turn-on fluorescence signals of DMDAP/Apt-AuNP upon PDGFs binding, the approach is highly sensitive for the detection of PDGFs. The DMDAP/Apt-AuNP probe specifically and sensitively detected PDGFs under optimal concentrations of salts and DMDAP. We also demonstrated that the Apt-AuNPs are effective selectors for enrichment of PDGF-AA from large-volume samples. The approach allows detection of PDGF-AA at a concentration down to 8 pM, showing better sensitivity than other signal aptamers. By conducting a competitive assay, we demonstrated the determination of PDGF receptor-alpha with LOD of 0.25 nM when using the DMDAP/Apt-AuNP as a probe.
    URI: http://pubs.acs.org/
    American Chemical Society
    http://nthur.lib.nthu.edu.tw/handle/987654321/40766
    Appears in Collections:[生醫工程與環境科學系] 期刊論文

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