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    National Tsing Hua University Institutional Repository > 生命科學院  > 生命科學系 > 期刊論文 >  Heparin binding stabilizes the membrane-bound form of cobra cardiotoxin

    Please use this identifier to cite or link to this item: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/48414

    Title: Heparin binding stabilizes the membrane-bound form of cobra cardiotoxin
    Authors: Shih-Che Sue;Kun-Yi Chien;Wei-Ning Huang;Joseph K. Abraham;Kuan-Ming Chen;Wen-guey Wu
    教師: 吳文桂
    Date: 2002
    Publisher: American Society for Biochemistry and Molecular Biology
    Relation: JOURNAL OF BIOLOGICAL CHEMISTRY,American Society for Biochemistry and Molecular Biology,Volume 277,Issue 4,JAN 25 2002,Pages 2666-2673
    Keywords: PHOSPHOLIPASE A(1)
    Abstract: It has been shown previously that the long chain fragments of heparin bind to the beta-strand cationic belt of the three-finger cobra cardiotoxin (or cytotoxin, CTX) and hence enhance its penetration into phospholipid monolayer under physiological ionic conditions. By taking lysophosphatidyleholine (LPC) micelles as a membrane model, we have shown by H-1 NAIR study that the binding of heparin-derived hexasaccharide (Hep-6) to CTX at the beta-strand region can induce conformational changes of CTX near its membrane binding loops and promote the binding activity of CTX toward LPC. The Fourier-transform infrared spectra and NAIR nuclear Overhauser effect of Hep-6(.)CTX and (CTXLPC)-L-. complex in aqueous buffer also supplemented the aforementioned observation. Thus, the detected conformational change may presumably be the result of structural coupling between the connecting loops and its P-strands. This is the first documentation of results showing how the association of hydrophilic carbohydrate molecules with amphiphilic proteins can promote hydrophobic protein-lipid interaction via the stabilization of its membrane-bound form. A similar mechanism involving tripartite interactions of heparin, protein, and lipid molecules may be operative near the extracellular matrix of cell membranes.
    URI: http://www.asbmb.org/
    Appears in Collections:[生命科學系] 期刊論文

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