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    National Tsing Hua University Institutional Repository > 生命科學院  > 生命科學系 > 期刊論文 >  Role of the linker region in the expression of Rhizopus oryzae glucoamylase

    Please use this identifier to cite or link to this item: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/48954

    Title: Role of the linker region in the expression of Rhizopus oryzae glucoamylase
    Authors: Shu-Chuan Lin;Wei-Ting Liu;Shi-Hwei Liu;Wei-I Chou;Bor-Kai Hsiung;I-Ping Lin;Chia-Chin Sheu;Margaret Dah-Tsyr Chang
    教師: 張大慈
    Date: 2007
    Publisher: BioMed Central
    Relation: BMC Biochemistry,BioMed Central,Volume 8,Issue 9,2007
    Keywords: Rhizopus oryzae
    Abstract: Background
    Rhizopus oryzae glucoamylase (RoGA) consists of three domains: an amino (N)-terminal raw starch-binding domain (SBD), a glycosylated linker domain, and a carboxy (C)-terminal catalytic domain. The 36-amino-acid linker region (residues 132–167) connects the two functional domains, but its structural and functional roles are unclear.
    To characterize the linker sequences of RoGA and its involvement in protein expression, a number of RoGA variants containing deletions and mutations were constructed and expressed in Saccharomyces cerevisiae. Deletion analyses demonstrate that the linker region, especially within residues 161 to 167, is required for protein expression. In addition, site-directed mutagenesis and deglycosylation studies reveal that the linker region of RoGA contains both N- and O-linked carbohydrate moieties, and the N-linked oligosaccharides play a major role in the formation of active enzyme. Although the linker segment itself appears to have no ordered secondary structural conformation, the flexible region indeed contributes to the stabilization of functional N- and C-terminal domains.
    Our data provide direct evidence that the length, composition, and glycosylation of the interdomain linker play a central role in the structure and function of RoGA.
    URI: http://www.biomedcentral.com/
    Appears in Collections:[生命科學系] 期刊論文

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