National Tsing Hua University Institutional Repository:Novel microchip for in situ TEM imaging of living organisms and bio-reactions in aqueous conditions
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    National Tsing Hua University Institutional Repository > 生命科學院  > 生命科學系 > 期刊論文 >  Novel microchip for in situ TEM imaging of living organisms and bio-reactions in aqueous conditions


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    题名: Novel microchip for in situ TEM imaging of living organisms and bio-reactions in aqueous conditions
    作者: Liu KL;Wu CC;Huang YJ;Peng HL;Chang HY;Chang P;Hsu L;Yew TR
    教師: 張晃猷
    日期: 2008
    出版者: Royal Society of Chemistry
    關聯: LAB ON A CHIP,Royal Society of Chemistry,Volume 8,Issue 11,2008,Pages 1915-1921
    关键词: SCANNING-ELECTRON-MICROSCOPY
    KLEBSIELLA-PNEUMONIAE
    ESCHERICHIA-COLI
    摘要: A novel and disposable microchip (K-kit) with SiO2 nano-membranes was developed and used as a specimen kit for in situ imaging of living organisms in an aqueous condition using transmission electron microscopy (TEM) without equipment modi. cation. This K-kit enabled the successful TEM observation of living Escherichia coli cells and the tellurite reduction process in Klebsiella pneumoniae. The K. pneumoniae and Saccharomyces cerevisiae can stay alive in K-kit after continuous TEM imaging for up to 14 s and 42 s, respectively. Besides, different tellurite reduction profiles in cells grown in aerobic and anaerobic environments can be clearly revealed. These results demonstrate that the K-kit developed in this paper can be useful for observing living organisms and monitoring biological processes in situ.
    URI: http://www.rsc.org/
    http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/49059
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