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    National Tsing Hua University Institutional Repository > 生命科學院  > 生命科學系 > 期刊論文 >  Characterization of Prenylcysteines That Interact with P-glycoprotein and Inhibit Drug Transport in Tumor Cells


    Please use this identifier to cite or link to this item: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/81421


    Title: Characterization of Prenylcysteines That Interact with P-glycoprotein and Inhibit Drug Transport in Tumor Cells
    Authors: Lili Zhang;Clifford W. Sachs;Hua-Wen Fu;Robert L. Fine;Patrick J. Casey
    教師: 傅化文
    Date: 1995
    Publisher: American Society for Biochemistry and Molecular Biology
    Relation: Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, Volume 270, Issue 39, SEP 1995, Pages 22859-22865
    Keywords: MULTIDRUG-RESISTANCE
    PRENYLATED PROTEINS
    A-FACTOR
    GENE
    CANCER
    EXPRESSION
    Abstract: Prenylcysteine methyl esters that represent the C-terminal structures of prenylated proteins demonstrate specific substrate-like interactions with P-glycoprotein (Zhang, L., Sachs, C. W., Fine, R. L., and Casey, P. J.(1994) J. Biol. Chem. 269, 15973-15976). The simplicity of these compounds provides a unique system for probing the structural specificity of P-glycoprotein substrates. We have further assessed the structural elements of prenylcysteines involved in the interaction with P-glycoprotein. Carboxyl group methylation, a modification in many prenylated proteins, plays an essential role of blocking the negative charge at the free carboxylate. Substitution of the methyl ester with a methyl amide or simple amide does not change the ability of the molecule to stimulate P-glycoprotein ATPase activity, but substitution with a glycine is not tolerated unless the carboxyl group of glycine is methylated. The presence of a nitrogen atom, which is found in many P-glycoprotein substrates and modifiers, is also essential for prenylcysteines to interact with P-glycoprotein. The structure at the nitrogen atom can, however, influence the type of interaction. Acetylation of the free amino group of prenylcysteine results in a significant loss in the ability of prenylcysteines to stimulate P-glycoprotein ATPase activity. Instead, certain acetylated prenylcysteines behave as inhibitors of this activity. In studies using MDR1-transfected human breast cancer cells, the acetylated prenylcysteine analogs inhibit P-glycoprotein-mediated drug transport and enhance the steady-state accumulation of [GraphicH]vinblastine, [GraphicH]colchicine, and [GraphicH]taxol. These inhibitors do not, however, affect drug accumulation in parental cells. These studies provide a novel approach for designing P-glycoprotein inhibitors that could prove effective in reversing the phenotype of multidrug resistance in tumor cells.
    Relation Link: http://www.asbmb.org/
    URI: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/81421
    Appears in Collections:[分子與細胞生物研究所] 期刊論文
    [生命科學系] 期刊論文

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