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    National Tsing Hua University Institutional Repository > 生命科學院  > 生命科學系 > 會議論文  >  The neutrophil-activating protein of Helicobacter pylori promotes myeloperoxidase release from human netruophils


    Please use this identifier to cite or link to this item: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/81436


    Title: The neutrophil-activating protein of Helicobacter pylori promotes myeloperoxidase release from human netruophils
    Authors: H.-W. Fu;C.-A. Wang;Y.-C. Liu;S.-Y. Du
    教師: 傅化文
    Date: 2008
    Publisher: Wiley-Blackwell
    Relation: FEBS Journal, Wiley-Blackwell, Volume 275, Issue Supplement s1, Special Issue: Abstracts of the 33rd FEBS Congress and 11th IUBMB Conference, PP5B-10, JUN 2008, Pages 99–437
    Abstract: Helicobacter pylori (H. pylori) is closely associated with chronic gastritis, peptic ulcer disease, and gastric adenocarcinoma and lymphoma. Helicobacter pylori neutrophil-activating protein (HP-NAP) is an important virulence factor of H. pylori for its pathogenesis. To investigate how HP-NAP activates human neutrophils, HP-NAP was expressed in Escherichia coli (E.coli), purified by the two sequential gel filtration chromatographies, and then subjected to filtration for the removal of endotoxin. The recombinant HP-NAP was able to stimulate human neutrophils to produce reactive oxygen species (ROS) as examined by luminol-enhanced chemiluminescent assay. HP-NAP can also promote the secretion of myeloperoxidase (MPO) in human neutrophils. The finding of this new function of HP-NAP in human neutrophils suggests that there should be more host immune responses of HP-NAP awaited to be explored.
    Relation Link: http://www.wiley.com/wiley-blackwell‎
    URI: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/81436
    Appears in Collections:[分子與細胞生物研究所] 會議論文
    [生命科學系] 會議論文

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