FEBS Journal, Wiley-Blackwell, Volume 275, Issue Supplement s1, Special Issue: Abstracts of the 33rd FEBS Congress and 11th IUBMB Conference, PP5B-10, JUN 2008, Pages 99–437
Helicobacter pylori (H. pylori) is closely associated with chronic gastritis, peptic ulcer disease, and gastric adenocarcinoma and lymphoma. Helicobacter pylori neutrophil-activating protein (HP-NAP) is an important virulence factor of H. pylori for its pathogenesis. To investigate how HP-NAP activates human neutrophils, HP-NAP was expressed in Escherichia coli (E.coli), puriﬁed by the two sequential gel filtration chromatographies, and then subjected to ﬁltration for the removal of endotoxin. The recombinant HP-NAP was able to stimulate human neutrophils to produce reactive oxygen species (ROS) as examined by luminol-enhanced chemiluminescent assay. HP-NAP can also promote the secretion of myeloperoxidase (MPO) in human neutrophils. The ﬁnding of this new function of HP-NAP in human neutrophils suggests that there should be more host immune responses of HP-NAP awaited to be explored.