English  |  正體中文  |  简体中文  |  Items with full text/Total items : 54367/62174 (87%)
Visitors : 9410399      Online Users : 84
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTHU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    National Tsing Hua University Institutional Repository > 生命科學院  > 生命科學系 > 期刊論文 >  Electrical stimulation promotes nerve growth factor-induced neurite outgrowth and signaling


    Please use this identifier to cite or link to this item: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/81438


    Title: Electrical stimulation promotes nerve growth factor-induced neurite outgrowth and signaling
    Authors: Yu-Jung Changa;Che-Ming Hsuc;Chia-Hua Lina;Michael Shiang-Cheng Luc;Linyi Chena.
    教師: 陳令儀
    Date: 2013
    Publisher: Elsevier
    Relation: Biochimica et Biophysica Acta (BBA) - General Subjects, Elsevier, Volume 1830, Issue 8, AUG 2013, Pages 4130-4136
    Keywords: Electrical stimulation
    Nerve growth factor
    Neurite outgrowth
    Abstract: Background
    Neurotrophins are important regulators for neural development and regeneration. Nerve growth factor (NGF) therapy has been tested in various models of neural injury and degeneration. However, whether NGF can reach target tissues and maintain effective concentration for a certain period of time remains uncertain. To facilitate neural regeneration, we investigate the possibility of combining NGF and electrical stimulation (ES) in promoting neurite outgrowth, an essential process during neural regeneration.

    Methods
    PC12 cells were seeded on collagen and indium tin oxide (ITO)-coated area on the transparent conductive devices. Cells were then subjected to the combination of ES and NGF treatment. Neurite outgrowth was compared.

    Results
    Our findings suggest that ES of 100 mV/mm together with NGF provides optimal effect on neurite outgrowth of PC12 cells. ES increases NGF-induced neurite length but reduces neurite branching, indicative of its primary effect on neurite elongation instead of initiation. One mechanism that ES enhances neurite outgrowth is through increasing NGF-induced phosphorylation of ERK1/2 (pERK1/2) and expression of Egr1 gene. ES has previously been demonstrated to increase the activity of protein kinase C (PKC). Our result indicates that activating PKC further increases NGF-induced pERK1/2 and thus neurite outgrowth.

    Conclusion
    It is likely that ES promotes NGF-induced neurite outgrowth through modulating the activity of ERK1/2.

    General significance
    Findings from this study suggest that combining ES and NGF provides a promising strategy for promoting neurite outgrowth.
    Relation Link: http://www.elsevier.com/
    URI: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/81438
    Appears in Collections:[腦科學研究中心] 期刊論文
    [分子醫學研究所] 期刊論文
    [生命科學系] 期刊論文

    Files in This Item:

    File SizeFormat
    2050132010013pdf.pdf824KbAdobe PDF627View/Open


    在NTHUR中所有的資料項目都受到原著作權保護,僅提供學術研究及教育使用,敬請尊重著作權人之權益。若須利用於商業或營利,請先取得著作權人授權。
    若發現本網站收錄之內容有侵害著作權人權益之情事,請權利人通知本網站管理者(smluo@lib.nthu.edu.tw),管理者將立即採取移除該內容等補救措施。

    SFX Query

    與系統管理員聯絡

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback