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    National Tsing Hua University Institutional Repository > 生命科學院  > 生命科學系 > 期刊論文 >  Electrical stimulation promotes nerve growth factor-induced neurite outgrowth and signaling

    Please use this identifier to cite or link to this item: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/81438

    Title: Electrical stimulation promotes nerve growth factor-induced neurite outgrowth and signaling
    Authors: Yu-Jung Changa;Che-Ming Hsuc;Chia-Hua Lina;Michael Shiang-Cheng Luc;Linyi Chena.
    教師: 陳令儀
    Date: 2013
    Publisher: Elsevier
    Relation: Biochimica et Biophysica Acta (BBA) - General Subjects, Elsevier, Volume 1830, Issue 8, AUG 2013, Pages 4130-4136
    Keywords: Electrical stimulation
    Nerve growth factor
    Neurite outgrowth
    Abstract: Background
    Neurotrophins are important regulators for neural development and regeneration. Nerve growth factor (NGF) therapy has been tested in various models of neural injury and degeneration. However, whether NGF can reach target tissues and maintain effective concentration for a certain period of time remains uncertain. To facilitate neural regeneration, we investigate the possibility of combining NGF and electrical stimulation (ES) in promoting neurite outgrowth, an essential process during neural regeneration.

    PC12 cells were seeded on collagen and indium tin oxide (ITO)-coated area on the transparent conductive devices. Cells were then subjected to the combination of ES and NGF treatment. Neurite outgrowth was compared.

    Our findings suggest that ES of 100 mV/mm together with NGF provides optimal effect on neurite outgrowth of PC12 cells. ES increases NGF-induced neurite length but reduces neurite branching, indicative of its primary effect on neurite elongation instead of initiation. One mechanism that ES enhances neurite outgrowth is through increasing NGF-induced phosphorylation of ERK1/2 (pERK1/2) and expression of Egr1 gene. ES has previously been demonstrated to increase the activity of protein kinase C (PKC). Our result indicates that activating PKC further increases NGF-induced pERK1/2 and thus neurite outgrowth.

    It is likely that ES promotes NGF-induced neurite outgrowth through modulating the activity of ERK1/2.

    General significance
    Findings from this study suggest that combining ES and NGF provides a promising strategy for promoting neurite outgrowth.
    Relation Link: http://www.elsevier.com/
    URI: http://nthur.lib.nthu.edu.tw/dspace/handle/987654321/81438
    Appears in Collections:[腦科學研究中心] 期刊論文
    [分子醫學研究所] 期刊論文
    [生命科學系] 期刊論文

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